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Construction of a topologically linked DNA network using DNA circles

Abstract

DNA catenanes, mechanically interlocked rings which provide unique properties based on its inseparability, are promising building blocks for nanostructures and molecular machines. Some of them form naturally, like the massive network kinetoplast DNA (kDNA) consisting of dsDNA circles resembling medieval hauberk. Here we forge a “DNA hauberk”, a novel ssDNA catenane. A linear ssDNA works as a scaffold to help construct the first layer of several interlocked DNA rings by enzymatic cyclization. Then strands of the next layer bind to its bottom and get cyclized. Two different layers are arranged alternately, each of which is composed of two different rings. Finally, we will get a brand new network made of DNA rings in this way. We use PAGE to check our works. Our results suggest a new model for constructing a topologically linked DNA network which can be used as a template to orient nano materials.

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